| First Author | Michelucci A | Year | 2019 |
| Journal | Elife | Volume | 8 |
| PubMed ID | 31657717 | Mgi Jnum | J:284518 |
| Mgi Id | MGI:6382238 | Doi | 10.7554/eLife.47576 |
| Citation | Michelucci A, et al. (2019) Transverse tubule remodeling enhances Orai1-dependent Ca(2+) entry in skeletal muscle. Elife 8:e47576 |
| abstractText | Exercise promotes the formation of intracellular junctions in skeletal muscle between stacks of sarcoplasmic reticulum (SR) cisternae and extensions of transverse-tubules (TT) that increase co-localization of proteins required for store-operated Ca(2+) entry (SOCE). Here, we report that SOCE, peak Ca(2+) transient amplitude and muscle force production during repetitive stimulation are increased after exercise in parallel with the time course of TT association with SR-stacks. Unexpectedly, exercise also activated constitutive Ca(2+) entry coincident with a modest decrease in total releasable Ca(2+) store content. Importantly, this decrease in releasable Ca(2+) store content observed after exercise was reversed by repetitive high-frequency stimulation, consistent with enhanced SOCE. The functional benefits of exercise on SOCE, constitutive Ca(2+) entry and muscle force production were lost in mice with muscle-specific loss of Orai1 function. These results indicate that TT association with SR-stacks enhances Orai1-dependent SOCE to optimize Ca(2+) dynamics and muscle contractile function during acute exercise. |
