| Primary Identifier | MGI:5443860 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | Parental ES cells were derived from B6.129S4-Krastm4Tyj mice. |
| molecularNote | The ES cells were targeted in the 3' UTR of the Col1a1 locus with a Flp-in construct containing a frt flanked PGK-Neo cassette followed by a Hygromicin gene lacking a proper ATG. Correctly targeted ES cells were selected on basis of Neomycin resistance. The targeted Col1a1 locus can later be used as a docking site for the introduction of a plasmid, minimally containing the desired transgene sequence and an frt site preceded by a constitutive promoter and an ATG initiation codon. |
