| First Author | Nakatani T | Year | 2015 |
| Journal | EMBO Rep | Volume | 16 |
| Issue | 5 | Pages | 582-9 |
| PubMed ID | 25694116 | Mgi Jnum | J:222880 |
| Mgi Id | MGI:5645854 | Doi | 10.15252/embr.201439427 |
| Citation | Nakatani T, et al. (2015) Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced gammaH2AX accumulation. EMBO Rep 16(5):582-9 |
| abstractText | In the mouse zygote, Stella/PGC7 protects 5-methylcytosine (5mC) of the maternal genome from Tet3-mediated oxidation to 5-hydroxymethylcytosine (5hmC). Although ablation of Stella causes early embryonic lethality, the underlying molecular mechanisms remain unknown. In this study, we report impaired DNA replication and abnormal chromosome segregation (ACS) of maternal chromosomes in Stella-null embryos. In addition, phosphorylation of H2AX (gammaH2AX), which has been reported to inhibit DNA replication, accumulates in the maternal chromatin of Stella-null zygotes in a Tet3-dependent manner. Cell culture assays verified that ectopic appearance of 5hmC induces abnormal accumulation of gammaH2AX and subsequent growth retardation. Thus, Stella protects maternal chromosomes from aberrant epigenetic modifications to ensure early embryogenesis. |
