| Primary Identifier | MGI:5448560 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Mrgprb4 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The entire open reading frame of the gene, encoded by a single exon, was replaced with a tTA-IRES-hPLAP-ACN targeting cassette. Tetracycline transactivator (Tta), an internal ribosome entry site (IRES), and human placental alkaline phosphatase (hPLAP) are fused with a self-excising loxP-flanked pol-II promoter-neomycin resistance cassette (ACN). Cre-mediated recombination removed the neo cassette. |
