| First Author | Kellogg CM | Year | 2023 |
| Journal | iScience | Volume | 26 |
| Issue | 12 | Pages | 108413 |
| PubMed ID | 38058312 | Mgi Jnum | J:359686 |
| Mgi Id | MGI:7564931 | Doi | 10.1016/j.isci.2023.108413 |
| Citation | Kellogg CM, et al. (2023) Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age. iScience 26(12):108413 |
| abstractText | Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (<2 m.o.) Tam induction, but age-related neurodegeneration and aging studies can require Cre induction in older mice (>12 m.o.). While anecdotally reported as problematic, there are no published comparisons of Tam-mediated Cre induction at early and late ages. Here, microglial-specific Cx3cr1(creERT2) mice were crossed to a floxed NuTRAP reporter to compare Cre induction at early (3-6 m.o.) and late (20 m.o.) ages. Specificity and efficiency of microglial labeling at 21-22 m.o. were identical in mice induced with Tam at early and late ages. Age-related microglial translatomic changes were also similar regardless of Tam induction age. Each Cre and flox mouse line should be independently validated, however, these findings demonstrate that Tam-mediated Cre induction can be performed even into older mouse ages and should be generalizable to other inducible Cre models. |
