| First Author | Lin JC | Year | 2014 |
| Journal | Biochim Biophys Acta | Volume | 1843 |
| Issue | 4 | Pages | 769-79 |
| PubMed ID | 24389249 | Mgi Jnum | J:210858 |
| Mgi Id | MGI:5571985 | Doi | 10.1016/j.bbamcr.2013.12.018 |
| Citation | Lin JC, et al. (2014) Emerging role for RNA binding motif protein 4 in the development of brown adipocytes. Biochim Biophys Acta 1843(4):769-79 |
| abstractText | RNA-binding motif protein 4 (RBM4) reportedly reprograms the tissue-specific splicing network which modulates the development of muscles and pancreatic beta-islets. Herein, we report that Rbm4a(-/-) mice exhibited hyperlipidemia accompanied with reduced mass of interscapular brown adipose tissue (iBAT). Elevated RBM4a led to the isoform shift of IR, Ppar-gamma, and Pref-1 genes which play pivotal roles in the different stages of adipogenesis. Overexpression of RBM4a enhanced the mitochondrial activity of brown adipocyte-like lineage in the presence of uncoupling agent. RBM4a-ablated adipocytes inversely exhibited impaired development and inefficient energy expenditure. Intriguingly, overexpressed RBM4a induced the expression of brown adipocyte-specific factors (Prdm16 and Bmp7) in white adipocyte-like lineage, which suggested the potential action of RBM4a on the white-to-brown trans-differentiation of adipocytes. In differentiating adipocytes, RBM4a constituted a feed-forward circuit through autoregulating the splicing pattern of its own transcript. Based on these results, we propose the emerging role of RBM4 in regulating the adipocyte-specific splicing events and transcription cascade, which subsequently facilitate the development and function of brown adipocyte-like cells. |
