| Primary Identifier | MGI:5464909 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Sox2 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NTac)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The COIN module (L71 loxP site, inverted polyadenylation sequence, inverted EGFP, L66 loxP site and FRT-flanked neo cassette) was inserted after the thirtieth nucleotide of the coding exon. This insertion divides the CpG island. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination restored the orientation of the EGFP to allow for expression. Western blot analysis confirmed a reduction in protein expression in heterozygous samples. |
