| Primary Identifier | MGI:5467924 | Allele Type | Targeted |
| Attribute String | RMCE-ready, Transactivator | Gene | Ptf1a |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked targeting vector containing a reverse tetracycline transactivator (rtTA) in the 5' UTR and a frt-flanked hygromycin cassette downstream of exon 2 was inserted into the loxed cassette acceptor (LCA) allele by recombinant mediated cassette exchange (RMCE). rtTA is expressed under control of the endogenous Ptf1a promoter. The hygromycin cassette was removed by a flp mediated recombination. |
