| Primary Identifier | MGI:5469577 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Pik3ca |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was in upstream of the wild-type exon 20, which was followed by a polyadenylation sequence, an FRT-flanked neo cassette, 4x transcriptional STOP, a loxP site and a modified exon 20. The modified exon 20 contained an A to G transition results in the amino acid substitution of arginine for histidine at position 1047 (H1047R). Flp-mediated recombination removed the FRT-flanked neo cassette. Cre-mediated recombination is required to remove the wild-type exon 20 and allow for expression of the modified exon 20. |
