| Primary Identifier | MGI:5473008 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Mustn1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Mutant cell line EPD0917_3_F04 was used to create mutant mice which demonstrated germline transmission. |
| molecularNote | The L1L2_Pgk_P cassette was inserted at position 30602117 of Chromosome 14 upstream of the critical exon 2 and part of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 and part of exon 3 at position 30603225. The critical exon 2 and part of exon 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resuts in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Although homozygous aorta and skeletal muscle show a more than 99.9% reduction in transcript levels, under certain conditions such as proinflammatory stimuli (BaCl2-mediated muscle injurgy-regeneration), an increase in transcript expression is seen indicating that the artificial splice acceptor site is not 100% efficient and allows for gene expression. |
