| Primary Identifier | MGI:5475555 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | (129-Hprt1<b-m3> x C57BL/6)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This single-copy, targeted insertion into the Hprt locus consists of the macrophage-specific mouse Cd68 promoter followed by a cDNA encoding the human B cell leukemia/lymphoma 2 protein and the mouse EF1 polyadenylation signal; the vector also contains sequence that repairs an inactivating deletion in the Hprt gene of the ES cell line. Human BCL2 mRNA and protein are detected by reverse transcriptase/polymerase chain reaction (RT-PCR) and flow cytometry, respectively, of thioglycollate-elicited peritoneal macrophages from transgenic mice. |
