Primary Identifier | MGI:5473958 | Allele Type | Targeted |
Attribute String | Inducible, Inserted expressed sequence, RMCE-ready | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Induced With | doxycycline/tetracycline | Is Recombinase | false |
Is Wild Type | false |
description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae (J:159351). |
molecularNote | RMCE was used to insert a human beta-catenin sequence containing an S33Y codon substitution under the control of a tetO and a minimal promoter. Insertion was in the 3' non coding region. |