| Primary Identifier | MGI:5476515 | Allele Type | Targeted |
| Attribute String | Reporter, RMCE-ready | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was generate from Gt(ROSA)26Sortm1Mgn. |
| molecularNote | This allele was created by RMCE in Gt(ROSA)26Sortm1Mgn. The original selections cassettes (puromycin N-acetyltransferase and a truncated form of HSV1 thymidine kinase and EM7-driven neomycin resistance) were replaced by a cassette composed of ROSA26 upstream sequence deleted from the start codon to -60, 5X direct repeat of a DR5 (AGGTCAccaggAGGTCA) which is a consensus RARE controlling expression of the CFP reporter. An upstream FLP flanked hygromycin selection cassette was subsequently removed by flp recombinase. |
