| First Author | Patterson GH | Year | 2002 |
| Journal | Science | Volume | 297 |
| Issue | 5588 | Pages | 1873-7 |
| PubMed ID | 12228718 | Mgi Jnum | J:195187 |
| Mgi Id | MGI:5476712 | Doi | 10.1126/science.1074952 |
| Citation | Patterson GH, et al. (2002) A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297(5588):1873-7 |
| abstractText | We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange. |
