| Primary Identifier | MGI:5478606 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)(J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | RMCE on KH2 cells inserted a cassette into the 3' UTR. The cassette contains the tetracycline responsive element (tetO) driving inducible expression of a C-terminal HA-tagged Yod1) with nucleotide substitutions that result in the amino acid substitution of serine for cysteine at position 160 (C160S). This mutation is expected to produce a catalytically inactivated product. |
