| Primary Identifier | MGI:5484764 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Serpinb5 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was into intron 3. An FRT-flanked neo with a 3' loxP was into intron 4. Cre-mediated recombination removed the neo cassette and exon 4. RNA analysis of mammary tissue form homozygous mice detects only a mutant truncated RNA product. Immunoblot and immunohistochemical analyses confirmed the absence of protein in epidermis from homozygous mice. Depending on the antibody used a 42kDa species is detected in the thymus and prostate; however, this is thought to be the result of cross-reaction with of clade B serpins. |
