| Primary Identifier | MGI:5491600 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Igs6 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the Iis6 locus located about 16 kb downstream of exon 1 of the Rab10 gene at 1.71 cM. Cre-mediated recombination removed the neo cassette. |
