| Primary Identifier | MGI:5495041 | Allele Type | Targeted |
| Attribute String | Reporter, RMCE-ready | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The vector was made on a backbone of a basal construct containing 5.166 kb from Rosa26 region that was absent in the Rosa26LCA allele, tandem Lox71/Lox2272 sites and a flrted (flanked by FRT)Pgk-Neo cassette for positive selection of ES cells after RMCE. Within the Rosa26 gene sequences the region from -228 to +81 were replaced by a Wnt response element (7xTcf/Lef binding site) fused to a TATA-Cerulean (CFP) reporter. The chimeric mice were crossed with Flpe mice to remove the neomycin resistance gene. |
