| Primary Identifier | MGI:7860792 | Allele Type | Endonuclease-mediated |
| Attribute String | Inserted expressed sequence | Gene | Prnp |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The open reading frame, which is located entirely within the third exon, was replaced with the bank vole prion gene containing isoleucine at polymorphic codon 109 (I109) and an FRT-flanked neomycin selection cassette via CRISPR/Cas9 technology. Flp-mediated recombination removed the neomycin selection cassette. The I109 polymorphic variant results in more rapid development of spontaneous disease than the M109 polymorphic variant. |
