| Primary Identifier | MGI:5501339 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Lman1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell line EPD0807_3_B06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:213133. |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 66131224 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 66132252. The critical exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can is created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
