| Primary Identifier | MGI:7860794 | Allele Type | Endonuclease-mediated |
| Attribute String | Inserted expressed sequence | Gene | Prnp |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The open reading frame, which is located entirely within the third exon, was replaced with the bank vole prion gene containing isoleucine at polymorphic codon 109 (I109), a glutamate to lysine substitution at position 200 (p.E200K) and an FRT-flanked neomycin selection cassette via CRISPR/Cas9 technology. Flp-mediated recombination removed the neomycin selection cassette. The E200K mutation causes familial Creutzfeldt-Jakob disease when paired with either methionine or valine at polymorphic codon 129 in human Prnp. |
