| Primary Identifier | MGI:5502307 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Rasa3 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The HTGRS1006_A_4_H03 targeting vector was obtained from The International Knockout Mouse Consortium by The Jackson Laboratory. The L1L2_Pgk_P cassette was inserted at position 13615101 of Chromosome 8 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 13614300. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision removed the critical sequence and the neomycin selection cassette yielding a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
