| Primary Identifier | MGI:5501471 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sec24c |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0241_2_A11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232065 |
| molecularNote | The L1L2_Pgk_P cassette was inserted at position 20679037 of Chromosome 14 upstream of exon 3. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 20679926. A null allele was created by flp and cre recombinase expression in mice, resulting in the deletion of the lacZ sequences, the neomycin selection cassette and exon 3. |
