Primary Identifier | MGI:5505280 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Ralb |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette was inserted downstream of exon 2. Flp-mediated recombination removed the neomycin resistance cassette and left exon 2 floxed. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts, kidney, lung and testis. |