| First Author | Cha SJ | Year | 2016 |
| Journal | J Exp Med | Volume | 213 |
| Issue | 10 | Pages | 2099-112 |
| PubMed ID | 27551151 | Mgi Jnum | J:237037 |
| Mgi Id | MGI:5810802 | Doi | 10.1084/jem.20160059 |
| Citation | Cha SJ, et al. (2016) Identification of GAPDH on the surface of Plasmodium sporozoites as a new candidate for targeting malaria liver invasion. J Exp Med 213(10):2099-112 |
| abstractText | Malaria transmission begins when an infected mosquito delivers Plasmodium sporozoites into the skin. The sporozoite subsequently enters the circulation and infects the liver by preferentially traversing Kupffer cells, a macrophage-like component of the liver sinusoidal lining. By screening a phage display library, we previously identified a peptide designated P39 that binds to CD68 on the surface of Kupffer cells and blocks sporozoite traversal. In this study, we show that the P39 peptide is a structural mimic of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) on the sporozoite surface and that GAPDH directly interacts with CD68 on the Kupffer cell surface. Importantly, an anti-P39 antibody significantly inhibits sporozoite liver invasion without cross-reacting with mammalian GAPDH. Therefore, Plasmodium-specific GAPDH epitopes may provide novel antigens for the development of a prehepatic vaccine. |
