| Primary Identifier | MGI:5512720 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, No functional change, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to contain a CAG promoter, followed by a loxP-STOP-LoxP (LSL) sequence and an H2B-mCherry fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Red Fluorescent Protein gene) An AttB-PGKNeo-AttP cassette was placed downstream of the H2B-mCherry fusion protein. This entire construct was inserted into the Gt(ROSA)26Sor locus. PhiC31-mediated recombination removed the neomycin resistance cassette. |
