| Primary Identifier | MGI:5515616 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dact3 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 3. Cre-mediated recombination removed exon 2, exon 3 and the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. |