| Primary Identifier | MGI:5515790 | Allele Type | Targeted |
| Gene | Chuk | Transmission | Germline |
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 2 was replaced with a modified exon 2 in which nucleotide substitutions (AAG to GCG) result in the amino acid substitution of alanine for lysine at position 44 (K44A). This mutation produces a kinase inactive protein. A floxed neomycin resistance cassette was inserted downstream of the modified exon 2. Western blot analysis confirmed reduced protein expression in splenocytes. |
