| Primary Identifier | MGI:5518653 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Itm2a |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 4. An IRES-nlacZ was inserted into exon 6. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exons 2 through 4 floxed but left the lacZ reporter intact. In situ hybridization confirmed reduced transcript expression in E11.5 embryos. |
