| Primary Identifier | MGI:5521293 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Piwil2 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 21. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 22. Flp-mediated recombination removed the neomycin resistance cassette and left exons 21 and 22 floxed. Cre-mediated recombination removed exons 21 and 22. Western blot analysis confirmed the absence of protein expression in the testis. |
