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Allele : Gt(ROSA)26Sor<tm2(CAG-FH*)Pjp> gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Patrick J Pollard
Primary Identifier  MGI:5518472 Allele Type  Targeted
Attribute String  Humanized sequence, Inserted expressed sequence, RMCE-ready Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  (C57BL/6J x 129S6/SvEvTac)F1
Is Recombinase  false Is Wild Type  false
description  The allele was made in IDG26.10-3 cells that carries Gt(ROSA)26Sortm1Rkuhn.
molecularNote  Recombinase mediated cassette exchange replaced the hygromycin resistance casssette in Gt(ROSA)26Sortm1Rkuhn with a neomycin resistance cassette, polyadenylation sequence, FRT site and a C-terminally V5-tagged human cDNA lacking the mitochondrial leader sequence (-MLS; FHcys) with a polyadenylation sequence driven by a CAG promoter.
  • mutations:
  • Insertion
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1 Feature

Genome

1 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

5 Publication categories





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