| Primary Identifier | MGI:5523143 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Oxt |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert an IRES linked to cre recombinase, followed by a Frt-flanked kanamycin resistance gene downstream of the oxytocin (Oxt) stop codon. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to flp expressing mice to delete the kanamycin selection cassette. |
