Primary Identifier | MGI:5523786 | Allele Type | Transgenic |
Attribute String | Recombinase | Gene | Tg(Slc32a1-cre)65Kzy |
Strain of Origin | C57BL/6 | Is Recombinase | true |
Is Wild Type | false |
molecularNote | Red/ET recombination was used to insert the sequences encoding cre recombinase, the SV40 poly(A) signal, and a kanamycin resistance gene at the ATG (translational initiation site) of the Slc32a1 (Vgat or Viaat) locus contained in RP23-392-P11 BAC clone derived from C57BL/6 mouse genomic DNA. The purified construct was injected into pronuclei of C57BL/6 oocytes. Transgenic line 65 was used in further analyses. |