| Primary Identifier | MGI:5527249 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Igs11 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The mutagenic insertion and chromosome engineering resource (MICER) clone MHPP322c09 was used to insert a loxP site at the 3' end of the keratin type II cluster, downstream of the type I Keratin 18 (Krt18) gene. This clone contains a 6.9 kb insert spanning 101,876,016-101,882,964 bp on chromosome 15 (genome build unknown) and includes a puromycin resistance gene cassette, a loxP site, an HPRT mini-gene, and the agouti (A) coat color gene. The construct was transfected into AB2.2 ES cells carrying Krt1ctm1Tmm and double-targeted clones in cis were identified for blastocyst injection. Cre-mediated removal of both loxP sites can be used to delete all of the type II keratins and one keratin type I, Krt18. |
