| Primary Identifier | MGI:5529780 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Tfap2a |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | The authors indicate that removal of the neomycin cassette by Cre-mediated recombination does not alter gross morphology or lacZ expression pattern. (J:73813) |
| molecularNote | An IRES, lacZ and floxed neomycin resistance cassette were inserted into exon 7 via homologous recombination resulting in disruption of the DNA binding domain. Cre-mediated recombination removed the floxed neo cassette. The mutant transcript encodes the first 6 exons of the gene followed by the IRES-lacZ message. The pattern of lacZ expression in mutant animals is similar to that of the endogenous protein. |
