Primary Identifier | MGI:5532323 | Allele Type | Targeted |
Attribute String | Hypomorph | Gene | P3h1 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An H662A (histidine to alanine; CAT to GCT) mutation was introduced to a catalytic site encoded by exon 14 via a recombineering strategy. A loxP-flanked neomycin resistance selection cassette was placed upstream, in intron 13. Germ line, cre-mediated recombination removed the neomycin cassette. Please note that an error in the publication indicates that the mutation was introduced into exon 12, the correct exon is 14. |