| Primary Identifier | MGI:5532717 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nr6a1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0105_6_D03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:204241 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 38632589 of Chromosome 2 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 38633441. The critical exon 7 is thus flanked by loxP sites. Flp-mediated recombination removed the reporter and selection cassette. Cre-mediated recombination removed exon 7. |
