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Allele : Nkx2-2<tm4.1(cre/EGFP)Suss> NK2 homeobox 2; targeted mutation 4.1, Lori Sussel
Primary Identifier  MGI:5538250 Allele Type  Targeted
Attribute String  Null/knockout, Recombinase Gene  Nkx2-2
Transmission  Germline Strain of Origin  129S6/SvEvTac
Is Recombinase  true Is Wild Type  false
molecularNote  A cre/EGFP fusion gene and Frt-flanked hygro selection cassette were inserted using recombineering into an Nkx2-2 basal exchange vector such that cre/EGFP was fused in-frame with the ATG start codon, replacing all but 25 base pairs of the first coding exon. This vector was co-injected with a cre-expression vector into ES cells containing an Nkx2-2 (loxed cassette acceptor) allele. Correctly exchanged ES clones were used to produce chimeras which were bred to B6 mice to verify germline transmission. Animals were bred to ACTB-Flpe mice to excise the hygromycin marker.
  • mutations:
  • Insertion,
  • Intragenic deletion
  • synonyms:
  • Nkx2.2:Cre-EGFP,
  • Nkx2.2<Cre>,
  • Nkx2.2<Cre>,
  • Nkx2.2:Cre-EGFP
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

1 Driven By

13 Publication categories





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