| Primary Identifier | MGI:5543602 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Sox17 |
| Transmission | Germline | Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A targeting vector was designed to delete the translational stop codon of Sox17 and to introduce the codon-optimized cre fused to the triple-mutant (G400V, M543A and L544A) human estrogen receptor ligand binding domain (ERT2) sequence preceded by a viral 2A peptide sequence into exon 5. Homologous recombination in ES cells integrated the cre/ERT2 cassette and Frt-flanked neomycin marker so that the targeted Sox17 allele will produce an bicistronic mRNA that will be co-translationally cleaved into equimolar amounts of Sox17 and cre. Chimeras were crossed to a Flp deleter line to remove the neo selection marker. |
