| Primary Identifier | MGI:5547589 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dnaaf4 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exons 2 through 4 floxed. Western blot analysis confirmed the absence of protein expression in the brain and lung. |
