| First Author | D'Angelo G | Year | 2013 |
| Journal | Nature | Volume | 501 |
| Issue | 7465 | Pages | 116-20 |
| PubMed ID | 23913272 | Mgi Jnum | J:205015 |
| Mgi Id | MGI:5543874 | Doi | 10.1038/nature12423 |
| Citation | D'Angelo G, et al. (2013) Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi. Nature 501(7465):116-20 |
| abstractText | Newly synthesized proteins and lipids are transported across the Golgi complex via different mechanisms whose respective roles are not completely clear. We previously identified a non-vesicular intra-Golgi transport pathway for glucosylceramide (GlcCer)--the common precursor of the different series of glycosphingolipids-that is operated by the cytosolic GlcCer-transfer protein FAPP2 (also known as PLEKHA8) (ref. 1). However, the molecular determinants of the FAPP2-mediated transfer of GlcCer from the cis-Golgi to the trans-Golgi network, as well as the physiological relevance of maintaining two parallel transport pathways of GlcCer--vesicular and non-vesicular--through the Golgi, remain poorly defined. Here, using mouse and cell models, we clarify the molecular mechanisms underlying the intra-Golgi vectorial transfer of GlcCer by FAPP2 and show that GlcCer is channelled by vesicular and non-vesicular transport to two topologically distinct glycosylation tracks in the Golgi cisternae and the trans-Golgi network, respectively. Our results indicate that the transport modality across the Golgi complex is a key determinant for the glycosylation pattern of a cargo and establish a new paradigm for the branching of the glycosphingolipid synthetic pathway. |
