| Primary Identifier | MGI:5544728 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Plekha8 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 4. An FRT-flanked splice acceptor IRES beta-geo polyadenylation sequence with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the beta-geo cassette and left exon 4 floxed. Cre-mediated recombination removed exon 4. Western blot analysis confirmed the absence of protein expression in testes and kidney. |