| Primary Identifier | MGI:5545787 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Rnf7 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0057_2_C04 was successfully used to make chimeric mice. Germline transmission was accomplished. J:205551 |
| molecularNote | An FRT-flanked cassette with a splice acceptor, lacZ gene, neomycin resistance gene and polyadenylation sequence (en2SA-LacZ-neo-pA) followed by a 3' loxP site was inserted upstream of the second coding exon. An additional loxP site was inserted downstream of the second coding exon. Flp-mediated recombination removed the lacZ/neomycin resistance cassette and left the second coding exon floxed. |
