| Primary Identifier | MGI:5550361 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Ghr |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell line EPD0125_5_H05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:206358 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 3376725 of Chromosome 15 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 3377560. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. |
