Primary Identifier | MGI:5559221 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Slx1b |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin resistance cassette with an IRES and a 5' loxP site was inserted upstream of the coding sequence in exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the coding sequence in exon 1 and the selection cassette. Western blot analysis confirmed the absence of protein expression in testis and mouse embryonic fibroblasts. |