| First Author | Yoshimi K | Year | 2021 |
| Journal | Lab Invest | Volume | 101 |
| Issue | 1 | Pages | 125-135 |
| PubMed ID | 32892213 | Mgi Jnum | J:339595 |
| Mgi Id | MGI:7520739 | Doi | 10.1038/s41374-020-00482-5 |
| Citation | Yoshimi K, et al. (2021) Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo. Lab Invest 101(1):125-135 |
| abstractText | Although the Cre-loxP recombination system has been extensively used to analyze gene function in vivo, spatiotemporal control of Cre activity is a critical limitation for easy and precise recombination. Here, we established photoactivatable-Cre (PA-Cre) knock-in (KI) mice at a safe harbor locus for the spatial and temporal regulation of Cre recombinase activity. The mice showed whole-body Cre recombination activity following light exposure for only 1 h. Almost no leaks of Cre recombination activity were detected in the KI mice under natural light conditions. Spot irradiation could induce locus-specific recombination noninvasively, enabling us to compare phenotypes on the left and right sides in the same mouse. Furthermore, long-term irradiation using an implanted wireless LED substantially improved Cre recombination activity, especially in the brain. These results demonstrate that PA-Cre KI mice can facilitate the spatiotemporal control of genetic engineering and provide a useful resource to elucidate gene function in vivo with Cre-loxP. |
