| Primary Identifier | MGI:5556209 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(hs23-Ccre,-lacZ)1Fawa |
| Strain of Origin | Not Specified | Is Recombinase | true |
| Is Wild Type | false |
| description | Examples of reporter expression driven by this and other human enhancers in mouse embryos produced by transient transgenesis can be viewed at http://enhancer.lbl.gov. |
| molecularNote | The C-terminal fragment of cre recombinase (aa 60-343) fused to the intein-C peptide fragment (36 C terminal amino acids) of the DnaE split intein sequence was cloned into a vector containing the human enhancer 23 sequence (hs23) and an Hsp68 minimal promoter and lacZ reporter. The final construct included an IRES sequence separating the lacZ gene and Ccre-intein-C. Injection of the linearized construct into pronuclei was performed to generated transgenic founders. Line numbers were not identified in the publication. |
