| Primary Identifier | MGI:5559540 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Crh |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 1 of the Crh gene contained in a BAC using recombineering methods. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Crh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. |
