Primary Identifier | MGI:5560495 | Allele Type | Targeted |
Gene | Cdk5r1 | Transmission | Germline |
Strain of Origin | 129 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The deltap35-3xHA knock-in cassette encodes for deletion of the six amino acid residues adjacent to the calpain-cleavage site (delta93-98) and replaces the alanine residue in the cleavage site for leucine., The cassette was generated via two sequential recombination reactions. The final targeting vector contains this deltap35-3xHA knock-in cassette and a loxP site-flanked NEO cassette. Mutant mice were bred with Meox2-cre mice to remove the neo selection cassette. |