| Primary Identifier | MGI:5562924 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(8Defb1-Defb13)1Jrdo |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A MICER clone carrying exons 1 and 2 of the HPRT gene, a neo cassette, a loxP site and Tyrosinase gene was inserted downstream of Defb13 and a MICER clone carrying exons 3-9 of the HPRT gene, a puro cassette, a loxP site and Agouti gene was inserted upstream of Defb1. Cre mediated recombination removed a 175 kb region encompassing 9 beta defensin genes (Defb1, Defb50, Defb2, Defb10, Defb9, Defb11, Defb15, Defb35 and Defb13) and reconstituting an intact Hprt gene. RT-PCR analysis confirmed the absence of expression of the nine deleted genes in homozygous mice. |
